Matrix-induced fragmentation of P3′-N5′ phosphoramidate-containing DNA: high-throughput MALDI-TOF analysis of genomic sequence polymorphisms

Chemical and enzymatic approaches were used to produce polynucleotide fragments containing acid-labile internucleotide P3′-N5′ phosphoramidate bonds, either in a surface-bound form or in solution. The primer extension reaction utilizing 5′-amino-5′-deoxynucleoside 5′-triphosphates generates polynucl...

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Detalhes bibliográficos

Publicado no:	Nucleic Acids Res
Principais autores:	Shchepinov, Mikhail S., Denissenko, Mikhail F., Smylie, Kevin J., Wörl, Ralf J., Leppin, A. Lorieta, Cantor, Charles R., Rodi, Charles P.
Formato:	Artigo
Idioma:	Inglês
Publicado em:	Oxford University Press 2001
Assuntos:	Article
Acesso em linha:	https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC55906/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/11557819/ https://ncbi.nlm.nih.govhttps://doi.org/10.1093/nar/29.18.3864
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