Matrix-induced fragmentation of P3′-N5′ phosphoramidate-containing DNA: high-throughput MALDI-TOF analysis of genomic sequence polymorphisms

Chemical and enzymatic approaches were used to produce polynucleotide fragments containing acid-labile internucleotide P3′-N5′ phosphoramidate bonds, either in a surface-bound form or in solution. The primer extension reaction utilizing 5′-amino-5′-deoxynucleoside 5′-triphosphates generates polynucl...

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Hlavní autoři: Shchepinov, Mikhail S., Denissenko, Mikhail F., Smylie, Kevin J., Wörl, Ralf J., Leppin, A. Lorieta, Cantor, Charles R., Rodi, Charles P.
Médium: Artigo
Jazyk:Inglês
Vydáno: Oxford University Press 2001
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On-line přístup:https://ncbi.nlm.nih.gov/pmc/articles/PMC55906/
https://ncbi.nlm.nih.gov/pubmed/11557819
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