High-throughput PCR assay design for targeted resequencing using primerXL

BACKGROUND: Although the sequencing landscape is rapidly evolving and sequencing costs are continuously decreasing, whole genome sequencing is still too expensive for use on a routine basis. Targeted resequencing of only the regions of interest decreases both costs and the complexity of the downstre...

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Veröffentlicht in:BMC Bioinformatics
Hauptverfasser: Lefever, Steve, Pattyn, Filip, De Wilde, Bram, Coppieters, Frauke, De Keulenaer, Sarah, Hellemans, Jan, Vandesompele, Jo
Format: Artigo
Sprache:Inglês
Veröffentlicht: BioMed Central 2017
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Online Zugang:https://ncbi.nlm.nih.gov/pmc/articles/PMC5588703/
https://ncbi.nlm.nih.gov/pubmed/28877663
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1186/s12859-017-1809-3
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