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Plasmid transfection influences the readout of nonsense-mediated mRNA decay reporter assays in human cells

Messenger RNA (mRNA) turnover is a crucial and highly regulated step of gene expression in mammalian cells. This includes mRNA surveillance pathways such as nonsense-mediated mRNA decay (NMD), which assesses the fidelity of transcripts and eliminates mRNAs containing a premature translation terminat...

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Vydáno v:Sci Rep
Hlavní autoři: Gerbracht, Jennifer V., Boehm, Volker, Gehring, Niels H.
Médium: Artigo
Jazyk:Inglês
Vydáno: Nature Publishing Group UK 2017
Témata:
On-line přístup:https://ncbi.nlm.nih.gov/pmc/articles/PMC5587671/
https://ncbi.nlm.nih.gov/pubmed/28878343
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1038/s41598-017-10847-4
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