Optimized strategy for in vivo Cas9-activation in Drosophila

While several large-scale resources are available for in vivo loss-of-function studies in Drosophila, an analogous resource for overexpressing genes from their endogenous loci does not exist. We describe a strategy for generating such a resource using Cas9 transcriptional activators (CRISPRa). First...

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Vydáno v:Proc Natl Acad Sci U S A
Hlavní autoři: Ewen-Campen, Ben, Yang-Zhou, Donghui, Fernandes, Vitória R., González, Delfina P., Liu, Lu-Ping, Tao, Rong, Ren, Xingjie, Sun, Jin, Hu, Yanhui, Zirin, Jonathan, Mohr, Stephanie E., Ni, Jian-Quan, Perrimon, Norbert
Médium: Artigo
Jazyk:Inglês
Vydáno: National Academy of Sciences 2017
Témata:
Biological Sciences
On-line přístup:https://ncbi.nlm.nih.gov/pmc/articles/PMC5584449/
https://ncbi.nlm.nih.gov/pubmed/28808002
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1073/pnas.1707635114
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