Single step generation of protein arrays from DNA by cell-free expression and in situ immobilisation (PISA method)
We describe a format for production of protein arrays termed ‘protein in situ array’ (PISA). A PISA is rapidly generated in one step directly from PCR-generated DNA fragments by cell-free protein expression and in situ immobilisation at a surface. The template for expression is DNA encoding individu...
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| Publicado no: | Nucleic Acids Res |
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| Principais autores: | , |
| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
Oxford University Press
2001
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| Assuntos: | |
| Acesso em linha: | https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC55838/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/11470888/ https://ncbi.nlm.nih.govhttps://doi.org/10.1093/nar/29.15.e73 |
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