Reproducible and inexpensive probe preparation for oligonucleotide arrays

We present a new protocol for the preparation of nucleic acids for microarray hybridization. DNA is fragmented quantitatively and reproducibly by using a hydroxyl radical-based reaction, which is initiated by hydrogen peroxide, iron(II)-EDTA and ascorbic acid. Following fragmentation, the nucleic ac...

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Detalhes bibliográficos

Publicado no:	Nucleic Acids Res
Principais autores:	Zhang, Yuzhi, Price, Brendan D., Tetradis, Sotirios, Chakrabarti, Subrata, Maulik, Gautam, Makrigiorgos, G. Mike
Formato:	Artigo
Idioma:	Inglês
Publicado em:	Oxford University Press 2001
Assuntos:	NAR Methods Online
Acesso em linha:	https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC55790/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/11433042/ https://ncbi.nlm.nih.govhttps://doi.org/10.1093/nar/29.13.e66
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