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Detection of mitochondrial single nucleotide polymorphisms using a primer elongation reaction on oligonucleotide microarrays

We have developed a novel allele-specific primer elongation protocol using a DNA polymerase on oligonucleotide chips. Oligonucleotide primers carrying polymorphic sites at their free 3́end were covalently bound to glass slides. The generation of single-stranded targets of genomic DNA containing sing...

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Detalhes bibliográficos
Main Authors: Erdogan, Fikret, Kirchner, Roland, Mann, Wolfgang, Ropers, Hans-Hilger, Nuber, Ulrike A.
Formato: Artigo
Idioma:Inglês
Publicado em: Oxford University Press 2001
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Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC31297/
https://ncbi.nlm.nih.gov/pubmed/11266571
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