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A simple method to measure CLOCK-BMAL1 DNA binding activity in tissue and cell extracts

The proteins CLOCK and BMAL1 form a heterodimeric transcription factor essential to circadian rhythms in mammals.  Daily rhythms of CLOCK-BMAL1 DNA binding activity are known to oscillate with target gene expression in vivo. Here we present a highly sensitive assay that recapitulates native CLOCK-BM...

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Vydáno v:F1000Res
Hlavní autoři: Gillessen, Maud, Kwak, Pieter Bas, Tamayo, Alfred
Médium: Artigo
Jazyk:Inglês
Vydáno: F1000Research 2017
Témata:
On-line přístup:https://ncbi.nlm.nih.gov/pmc/articles/PMC5580408/
https://ncbi.nlm.nih.gov/pubmed/28928952
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.12688/f1000research.11685.2
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