Measurement of steady-state kinetic parameters for DNA unwinding by the bacteriophage T4 Dda helicase: use of peptide nucleic acids to trap single-stranded DNA products of helicase reactions
Measurement of steady-state rates of unwinding of double-stranded oligonucleotides by helicases is hampered due to rapid reannealing of the single-stranded DNA products. Including an oligonucleotide in the reaction mixture which can hybridize with one of the single strands can prevent reannealing. H...
Wedi'i Gadw mewn:
| Cyhoeddwyd yn: | Nucleic Acids Res |
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| Prif Awduron: | , , , |
| Fformat: | Artigo |
| Iaith: | Inglês |
| Cyhoeddwyd: |
Oxford University Press
2001
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| Pynciau: | |
| Mynediad Ar-lein: | https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC55784/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/11433029/ https://ncbi.nlm.nih.govhttps://doi.org/10.1093/nar/29.13.2829 |
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