Measurement of steady-state kinetic parameters for DNA unwinding by the bacteriophage T4 Dda helicase: use of peptide nucleic acids to trap single-stranded DNA products of helicase reactions

Measurement of steady-state rates of unwinding of double-stranded oligonucleotides by helicases is hampered due to rapid reannealing of the single-stranded DNA products. Including an oligonucleotide in the reaction mixture which can hybridize with one of the single strands can prevent reannealing. H...

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Vydáno v:	Nucleic Acids Res
Hlavní autoři:	Nanduri, Bindu, Eoff, Robert L., Tackett, Alan J., Raney, Kevin D.
Médium:	Artigo
Jazyk:	Inglês
Vydáno:	Oxford University Press 2001
Témata:	Article
On-line přístup:	https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC55784/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/11433029/ https://ncbi.nlm.nih.govhttps://doi.org/10.1093/nar/29.13.2829
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