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Measurement of steady-state kinetic parameters for DNA unwinding by the bacteriophage T4 Dda helicase: use of peptide nucleic acids to trap single-stranded DNA products of helicase reactions

Measurement of steady-state rates of unwinding of double-stranded oligonucleotides by helicases is hampered due to rapid reannealing of the single-stranded DNA products. Including an oligonucleotide in the reaction mixture which can hybridize with one of the single strands can prevent reannealing. H...

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Main Authors: Nanduri, Bindu, Eoff, Robert L., Tackett, Alan J., Raney, Kevin D.
Formáid: Artigo
Teanga:Inglês
Foilsithe: Oxford University Press 2001
Ábhair:
Rochtain Ar Líne:https://ncbi.nlm.nih.gov/pmc/articles/PMC55784/
https://ncbi.nlm.nih.gov/pubmed/11433029
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