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Cpf1 proteins excise CRISPR RNAs from mRNA transcripts in mammalian cells
Cpf1 is a CRISPR-effector protein with greater specificity than Streptococcus pyogenes Cas9 (SpCas9) in genome-editing applications. Here we show that Lachnospiraceae bacterium (Lb) and Acidaminococus sp. (As) Cpf1 orthologs have RNase activities that can excise multiple CRISPR RNAs (crRNAs) from a...
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| Publicado no: | Nat Chem Biol |
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| Main Authors: | , , , , |
| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
2017
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| Assuntos: | |
| Acesso em linha: | https://ncbi.nlm.nih.gov/pmc/articles/PMC5577360/ https://ncbi.nlm.nih.gov/pubmed/28628097 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1038/nchembio.2410 |
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