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Versatile applications of transcriptional pulsing to study mRNA turnover in mammalian cells

Development of transcriptional pulsing approaches using the c-fos and Tet-off promoter systems greatly facilitated studies of mRNA turnover in mammalian cells. However, optimal protocols for these approaches vary for different cell types and/or physiological conditions, limiting their widespread app...

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Detalhes bibliográficos
Main Authors: Chen, Chyi-Ying A., Yamashita, Yukiko, Chang, Tsung-Cheng, Yamashita, Akio, Zhu, Wenmiao, Zhong, Zhenping, Shyu, Ann-Bin
Formato: Artigo
Idioma:Inglês
Publicado em: Cold Spring Harbor Laboratory Press 2007
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC1986818/
https://ncbi.nlm.nih.gov/pubmed/17728382
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1261/rna.663507
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