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Detection of the ubiquitinome in cells undergoing oncogene-induced senescence
Senescent cells exhibit dramatic changes in protein post-translational modifications. Here, we describe a method, stable isotope labeling with amino acids in cell culture (SILAC) coupled to liquid chromatography tandem mass spectrometry (LC-MS/MS), to identify changes in the ubiquitinome in cells th...
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| Publicado en: | Methods Mol Biol |
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| Main Authors: | , , , , |
| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado: |
2017
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| Assuntos: | |
| Acceso en liña: | https://ncbi.nlm.nih.gov/pmc/articles/PMC5572468/ https://ncbi.nlm.nih.gov/pubmed/27812874 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1007/978-1-4939-6670-7_12 |
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