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Construction and in vivo assembly of a catalytically proficient and hyperthermostable de novo enzyme
Although catalytic mechanisms in natural enzymes are well understood, achieving the diverse palette of reaction chemistries in re-engineered native proteins has proved challenging. Wholesale modification of natural enzymes is potentially compromised by their intrinsic complexity, which often obscure...
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| Publicado no: | Nat Commun |
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| Main Authors: | , , , , , , , , , , , , , , , |
| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
Nature Publishing Group UK
2017
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| Assuntos: | |
| Acesso em linha: | https://ncbi.nlm.nih.gov/pmc/articles/PMC5572459/ https://ncbi.nlm.nih.gov/pubmed/28842561 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1038/s41467-017-00541-4 |
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