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Construction and in vivo assembly of a catalytically proficient and hyperthermostable de novo enzyme

Although catalytic mechanisms in natural enzymes are well understood, achieving the diverse palette of reaction chemistries in re-engineered native proteins has proved challenging. Wholesale modification of natural enzymes is potentially compromised by their intrinsic complexity, which often obscure...

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Detalhes bibliográficos
Publicado no:Nat Commun
Main Authors: Watkins, Daniel W., Jenkins, Jonathan M. X., Grayson, Katie J., Wood, Nicola, Steventon, Jack W., Le Vay, Kristian K., Goodwin, Matthew I., Mullen, Anna S., Bailey, Henry J., Crump, Matthew P., MacMillan, Fraser, Mulholland, Adrian J., Cameron, Gus, Sessions, Richard B., Mann, Stephen, Anderson, J. L. Ross
Formato: Artigo
Idioma:Inglês
Publicado em: Nature Publishing Group UK 2017
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Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC5572459/
https://ncbi.nlm.nih.gov/pubmed/28842561
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1038/s41467-017-00541-4
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