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Four Inserts within the Catalytic Domain Confer Extra Stability and Activity to Hyperthermostable Pyrolysin from Pyrococcus furiosus

Pyrolysin from the hyperthermophilic archaeon Pyrococcus furiosus is the prototype of the pyrolysin family of the subtilisin-like serine protease superfamily (subtilases). It contains four inserts (IS147, IS29, IS27, and IS8) of unknown function in the catalytic domain. We performed domain deletions...

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Detalhes bibliográficos
Publicado no:Appl Environ Microbiol
Main Authors: Gao, Xiaowei, Zeng, Jing, Yi, Huawei, Zhang, Fang, Tang, Bing, Tang, Xiao-Feng
Formato: Artigo
Idioma:Inglês
Publicado em: American Society for Microbiology 2017
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Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC5311392/
https://ncbi.nlm.nih.gov/pubmed/28003199
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1128/AEM.03228-16
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