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Quantification of mRNA expression by competitive PCR using non-homologous competitors containing a shifted restriction site

Despite the recent introduction of real-time PCR methods, competitive PCR techniques continue to play an important role in nucleic acid quantification because of the significantly lower cost of equipment and consumables. Here we describe a shifted restriction-site competitive PCR (SRS-cPCR) assay ba...

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Detalhes bibliográficos
Main Authors: Watzinger, Franz, Hörth, Elfriede, Lion, Thomas
Formato: Artigo
Idioma:Inglês
Publicado em: Oxford University Press 2001
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC55722/
https://ncbi.nlm.nih.gov/pubmed/11376164
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