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Simple, sensitive and accurate method for the quantification of prothrombin mRNA by using competitive PCR.

A method for the quantification of prothrombin (PT) mRNA species in hepatic tissues of rats was developed with the use of competitive PCR. To validate the quantification approach, sequential dilutions of total RNA from one of the samples were reverse transcribed. Their equivalent volumes were amplif...

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Hlavní autoři: Grover, P K, Stapleton, A M, Miyazawa, K, Ryall, R L
Médium: Artigo
Jazyk:Inglês
Vydáno: 2001
Témata:
On-line přístup:https://ncbi.nlm.nih.gov/pmc/articles/PMC1221818/
https://ncbi.nlm.nih.gov/pubmed/11336642
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