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Protocol 4: Large-scale purification of SUMO modified proteins from S. pombe

Covalent protein modification by SUMO (sumoylation) regulates a broad spectrum of critical functions in eukaryotic cells; however, usually ≤ 1% of a given protein is modified due to the highly dynamic nature of sumoylation. As such, capturing and identifying sumoylated proteins is both important in...

Ausführliche Beschreibung

Gespeichert in:
Bibliographische Detailangaben
Veröffentlicht in:Cold Spring Harb Protoc
Hauptverfasser: Nie, Minghua, Boddy, Michael N.
Format: Artigo
Sprache:Inglês
Veröffentlicht: 2017
Schlagworte:
Online Zugang:https://ncbi.nlm.nih.gov/pmc/articles/PMC5570517/
https://ncbi.nlm.nih.gov/pubmed/28250213
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1101/pdb.prot091603
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