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Differential Salt Fractionation of Nuclei to Analyze Chromatin-associated Proteins from Cultured Mammalian Cells

Nucleosomes are the core units of cellular chromatin and are comprised of 147 base pairs (bp) of DNA wrapped around an octamer of histone proteins. Proteins such as chromatin remodelers, transcription factors, and DNA repair proteins interact dynamically with chromatin to regulate access to DNA, con...

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書目詳細資料
發表在:Bio Protoc
Main Authors: Herrmann, Christin, Avgousti, Daphne C., Weitzman, Matthew D.
格式: Artigo
語言:Inglês
出版: Bio-Protocol 2017
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在線閱讀:https://ncbi.nlm.nih.gov/pmc/articles/PMC5568101/
https://ncbi.nlm.nih.gov/pubmed/28845440
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.21769/BioProtoc.2175
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