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Differential Salt Fractionation of Nuclei to Analyze Chromatin-associated Proteins from Cultured Mammalian Cells

Nucleosomes are the core units of cellular chromatin and are comprised of 147 base pairs (bp) of DNA wrapped around an octamer of histone proteins. Proteins such as chromatin remodelers, transcription factors, and DNA repair proteins interact dynamically with chromatin to regulate access to DNA, con...

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Detalhes bibliográficos
Publicado no:Bio Protoc
Main Authors: Herrmann, Christin, Avgousti, Daphne C., Weitzman, Matthew D.
Formato: Artigo
Idioma:Inglês
Publicado em: Bio-Protocol 2017
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC5568101/
https://ncbi.nlm.nih.gov/pubmed/28845440
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.21769/BioProtoc.2175
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