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Differential Salt Fractionation of Nuclei to Analyze Chromatin-associated Proteins from Cultured Mammalian Cells

Nucleosomes are the core units of cellular chromatin and are comprised of 147 base pairs (bp) of DNA wrapped around an octamer of histone proteins. Proteins such as chromatin remodelers, transcription factors, and DNA repair proteins interact dynamically with chromatin to regulate access to DNA, con...

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Détails bibliographiques
Publié dans:Bio Protoc
Auteurs principaux: Herrmann, Christin, Avgousti, Daphne C., Weitzman, Matthew D.
Format: Artigo
Langue:Inglês
Publié: Bio-Protocol 2017
Sujets:
Accès en ligne:https://ncbi.nlm.nih.gov/pmc/articles/PMC5568101/
https://ncbi.nlm.nih.gov/pubmed/28845440
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.21769/BioProtoc.2175
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