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Facile single-stranded DNA sequencing of human plasma DNA via thermostable group II intron reverse transcriptase template switching

High-throughput single-stranded DNA sequencing (ssDNA-seq) of cell-free DNA from plasma and other bodily fluids is a powerful method for non-invasive prenatal testing, and diagnosis of cancers and other diseases. Here, we developed a facile ssDNA-seq method, which exploits a novel template-switching...

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Detalhes bibliográficos
Publicado no:Sci Rep
Main Authors: Wu, Douglas C., Lambowitz, Alan M.
Formato: Artigo
Idioma:Inglês
Publicado em: Nature Publishing Group UK 2017
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC5566474/
https://ncbi.nlm.nih.gov/pubmed/28827600
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1038/s41598-017-09064-w
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