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Murine Lymphocyte Labeling by (64)Cu-Antibody Receptor Targeting for In Vivo Cell Trafficking by PET/CT

This protocol illustrates the production of (64)Cu and the chelator conjugation/radiolabeling of a monoclonal antibody (mAb) followed by murine lymphocyte cell culture and (64)Cu-antibody receptor targeting of the cells. In vitro evaluation of the radiolabel and non-invasive in vivo cell tracking in...

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Bibliografiske detaljer
Udgivet i:J Vis Exp
Main Authors: Hoffmann, Sabrina H. L., Maurer, Andreas, Reck, Dorothea I., Reischl, Gerald, Pichler, Bernd J., Kneilling, Manfred, Griessinger, Christoph M.
Format: Artigo
Sprog:Inglês
Udgivet: MyJove Corporation 2017
Fag:
Online adgang:https://ncbi.nlm.nih.gov/pmc/articles/PMC5565140/
https://ncbi.nlm.nih.gov/pubmed/28518105
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.3791/55270
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