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Murine Lymphocyte Labeling by (64)Cu-Antibody Receptor Targeting for In Vivo Cell Trafficking by PET/CT
This protocol illustrates the production of (64)Cu and the chelator conjugation/radiolabeling of a monoclonal antibody (mAb) followed by murine lymphocyte cell culture and (64)Cu-antibody receptor targeting of the cells. In vitro evaluation of the radiolabel and non-invasive in vivo cell tracking in...
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| Pubblicato in: | J Vis Exp |
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| Autori principali: | , , , , , , |
| Natura: | Artigo |
| Lingua: | Inglês |
| Pubblicazione: |
MyJove Corporation
2017
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| Soggetti: | |
| Accesso online: | https://ncbi.nlm.nih.gov/pmc/articles/PMC5565140/ https://ncbi.nlm.nih.gov/pubmed/28518105 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.3791/55270 |
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