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Determination of High-affinity Antibody-antigen Binding Kinetics Using Four Biosensor Platforms
Label-free optical biosensors are powerful tools in drug discovery for the characterization of biomolecular interactions. In this study, we describe the use of four routinely used biosensor platforms in our laboratory to evaluate the binding affinity and kinetics of ten high-affinity monoclonal anti...
Gespeichert in:
| Veröffentlicht in: | J Vis Exp |
|---|---|
| Hauptverfasser: | , , , |
| Format: | Artigo |
| Sprache: | Inglês |
| Veröffentlicht: |
MyJove Corporation
2017
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| Schlagworte: | |
| Online Zugang: | https://ncbi.nlm.nih.gov/pmc/articles/PMC5564993/ https://ncbi.nlm.nih.gov/pubmed/28448040 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.3791/55659 |
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