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Determination of High-affinity Antibody-antigen Binding Kinetics Using Four Biosensor Platforms

Label-free optical biosensors are powerful tools in drug discovery for the characterization of biomolecular interactions. In this study, we describe the use of four routinely used biosensor platforms in our laboratory to evaluate the binding affinity and kinetics of ten high-affinity monoclonal anti...

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Bibliografski detalji
Izdano u:J Vis Exp
Glavni autori: Yang, Danlin, Singh, Ajit, Wu, Helen, Kroe-Barrett, Rachel
Format: Artigo
Jezik:Inglês
Izdano: MyJove Corporation 2017
Teme:
Online pristup:https://ncbi.nlm.nih.gov/pmc/articles/PMC5564993/
https://ncbi.nlm.nih.gov/pubmed/28448040
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.3791/55659
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