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Dense sgRNA Library Construction Using a Molecular Chipper Approach
Genetic screens using single-guide-RNA (sgRNA) libraries and CRISPR technology have been powerful to identify genetic regulators for both coding and noncoding regions of the genome. Interrogating functional elements in noncoding regions requires sgRNA libraries that are densely covering, and ideally...
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| Publicat a: | Bio Protoc |
|---|---|
| Autors principals: | , , |
| Format: | Artigo |
| Idioma: | Inglês |
| Publicat: |
Bio-Protocol
2017
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| Matèries: | |
| Accés en línia: | https://ncbi.nlm.nih.gov/pmc/articles/PMC5564686/ https://ncbi.nlm.nih.gov/pubmed/28835906 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.21769/BioProtoc.2373 |
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