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In vitro Assay to Measure DNA Polymerase β Nucleotide Insertion Coupled with the DNA Ligation Reaction during Base Excision Repair

We previously reported that oxidized nucleotide insertion by DNA polymerase β (pol β) can confound the DNA ligation step during base excision repair (BER) (Çağlayan et al., 2017). Here, we describe a method to investigate pol β nucleotide insertion coupled with DNA ligation, in the same reaction mix...

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Bibliographic Details
Published in:Bio Protoc
Main Authors: Çağlayan, Melike, Wilson, Samuel H.
Format: Artigo
Language:Inglês
Published: Bio-Protocol 2017
Subjects:
Online Access:https://ncbi.nlm.nih.gov/pmc/articles/PMC5564683/
https://ncbi.nlm.nih.gov/pubmed/28835905
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.21769/BioProtoc.2341
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