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His(6) tag-assisted chemical protein synthesis
To make more practical the total chemical synthesis of proteins by the ligation of unprotected peptide building blocks, we have developed a method to facilitate the isolation and handling of intermediate products. The synthetic technique makes use of a His(6) tag at the C terminus of the target poly...
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| Main Authors: | , |
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| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
National Academy of Sciences
2005
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| Assuntos: | |
| Acesso em linha: | https://ncbi.nlm.nih.gov/pmc/articles/PMC555969/ https://ncbi.nlm.nih.gov/pubmed/15784744 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1073/pnas.0407648102 |
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