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His(6) tag-assisted chemical protein synthesis

To make more practical the total chemical synthesis of proteins by the ligation of unprotected peptide building blocks, we have developed a method to facilitate the isolation and handling of intermediate products. The synthetic technique makes use of a His(6) tag at the C terminus of the target poly...

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Hlavní autoři: Bang, Duhee, Kent, Stephen B. H.
Médium: Artigo
Jazyk:Inglês
Vydáno: National Academy of Sciences 2005
Témata:
On-line přístup:https://ncbi.nlm.nih.gov/pmc/articles/PMC555969/
https://ncbi.nlm.nih.gov/pubmed/15784744
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1073/pnas.0407648102
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