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A new method to investigate the catalytic mechanism of YhdE pyrophosphatase by using a pyrophosphate fluorescence probe

YhdE is a Maf (multicopy associated filamentation) proteins from Escherichia coli which exhibits pyrophosphatase activity towards selected nucleotides, although its catalytic mechanism remains unclear. Herein we used a novel fluorescence probe (4-isoACBA–Zn(II) complex) to characterize the enzymatic...

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Detalhes bibliográficos
Publicado no:Sci Rep
Main Authors: Shen, Qingya, Tan, Hongwei, Xing, Guo-wen, Zheng, Jimin, Jia, Zongchao
Formato: Artigo
Idioma:Inglês
Publicado em: Nature Publishing Group UK 2017
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC5557916/
https://ncbi.nlm.nih.gov/pubmed/28811554
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1038/s41598-017-08368-1
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