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HiHiMap: single-cell quantitation of histones and histone posttranslational modifications across the cell cycle by high-throughput imaging

We describe High-throughput Histone Mapping (HiHiMap), a high-throughput imaging method to measure histones and histone posttranslational modifications (PTMs) in single cells. HiHiMap uses imaging-based quantification of DNA and cyclin A to stage individual cells in the cell cycle to determine the l...

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Publicat a:Mol Biol Cell
Autors principals: Zane, Linda, Chapus, Fleur, Pegoraro, Gianluca, Misteli, Tom
Format: Artigo
Idioma:Inglês
Publicat: The American Society for Cell Biology 2017
Matèries:
Accés en línia:https://ncbi.nlm.nih.gov/pmc/articles/PMC5555657/
https://ncbi.nlm.nih.gov/pubmed/28615324
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1091/mbc.E16-12-0870
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