Compartmentalized embryoid body culture for induction of spatially patterned differentiation

We developed a compartmentalized culture system of single embryoid bodies (EBs) utilizing a through-hole on a membrane to induce spatially patterned differentiation. An EB derived from mouse pluripotent stem cells was immobilized on the through-hole. By introducing a stem cell maintenance medium and...

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Bibliographic Details
Published in:Biomicrofluidics
Main Authors: Kaneda, Shohei, Kawada, Jiro, Akutsu, Hidenori, Ichida, Justin, Ikeuchi, Yoshiho, Fujii, Teruo
Format: Artigo
Language:Inglês
Published: AIP Publishing LLC 2017
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Online Access:https://ncbi.nlm.nih.gov/pmc/articles/PMC5552387/
https://ncbi.nlm.nih.gov/pubmed/28852428
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1063/1.4994989
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