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Use of gene transfer and a novel cosmid rescue strategy to isolate transforming sequences.
Mouse Lewis Lung tumor DNA was ligated to a cosmid containing a geneticin (G418)/kanamycin resistance gene and transferred into NIH3T3 cells. Recipient cells were first selected for geneticin resistance and subsequently for their ability to grow as a tumour when injected into nude mice. By repeating...
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| Publicat a: | EMBO J |
|---|---|
| Autors principals: | , , , , |
| Format: | Artigo |
| Idioma: | Inglês |
| Publicat: |
Nature Publishing Group
1985
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| Matèries: | |
| Accés en línia: | https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC554547/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/4054098/ https://ncbi.nlm.nih.govhttps://doi.org/10.1002/j.1460-2075.1985.tb03974.x |
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