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Use of gene transfer and a novel cosmid rescue strategy to isolate transforming sequences.

Mouse Lewis Lung tumor DNA was ligated to a cosmid containing a geneticin (G418)/kanamycin resistance gene and transferred into NIH3T3 cells. Recipient cells were first selected for geneticin resistance and subsequently for their ability to grow as a tumour when injected into nude mice. By repeating...

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Détails bibliographiques
Publié dans:EMBO J
Auteurs principaux: Brady, G, Funk, A, Mattern, J, Schütz, G, Brown, R
Format: Artigo
Langue:Inglês
Publié: Nature Publishing Group 1985
Sujets:
Accès en ligne:https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC554547/
https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/4054098/
https://ncbi.nlm.nih.govhttps://doi.org/10.1002/j.1460-2075.1985.tb03974.x
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