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Island rescue PCR: a rapid and efficient method for isolating transcribed sequences from yeast artificial chromosomes and cosmids.

The identification of transcripts from large genomic regions cloned in yeast artificial chromosomes (YACs) or cosmids continues to be a critical and often rate-limiting step in positional cloning of human disease genes. We have developed a PCR-based method for rapid and efficient generation of probe...

Disgrifiad llawn

Wedi'i Gadw mewn:
Manylion Llyfryddiaeth
Cyhoeddwyd yn:Proc Natl Acad Sci U S A
Prif Awduron: Valdes, J M, Tagle, D A, Collins, F S
Fformat: Artigo
Iaith:Inglês
Cyhoeddwyd: National Academy of Sciences 1994
Pynciau:
Mynediad Ar-lein:https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC43998/
https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/8202494/
https://ncbi.nlm.nih.govhttps://doi.org/10.1073/pnas.91.12.5377
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