A reliable assessment of 8-oxo-2-deoxyguanosine levels in nuclear and mitochondrial DNA using the sodium iodide method to isolate DNA

A major controversy in the area of DNA biochemistry concerns the actual in vivo levels of oxidative damage in DNA. We show here that 8-oxo-2-deoxyguanosine (oxo8dG) generation during DNA isolation is eliminated using the sodium iodide (NaI) isolation method and that the level of oxo8dG in nuclear DN...

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Detalhes bibliográficos
Main Authors: Hamilton, Michelle L., Guo, ZhongMao, Fuller, Clinton D., Van Remmen, Holly, Ward, Walter F., Austad, Steven N., Troyer, Dean A., Thompson, Ian, Richardson, Arlan
Formato: Artigo
Idioma:Inglês
Publicado em: Oxford University Press 2001
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Article
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC55450/
https://ncbi.nlm.nih.gov/pubmed/11353081
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