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Developing Fluorogenic Riboswitches for Imaging Metabolite Concentration Dynamics in Bacterial Cells

Genetically encoded small molecule sensors are important tools for revealing the dynamics of metabolites and other small molecules in live cells over time. We recently developed RNA-based sensors that exhibit fluorescence in proportion to a small molecule ligand. One class of these RNA-based sensors...

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Détails bibliographiques
Publié dans:Methods Enzymol
Auteurs principaux: Litke, Jacob L., You, Mingxu, Jaffrey, Samie R.
Format: Artigo
Langue:Inglês
Publié: 2016
Sujets:
Accès en ligne:https://ncbi.nlm.nih.gov/pmc/articles/PMC5540731/
https://ncbi.nlm.nih.gov/pubmed/27241761
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/bs.mie.2016.03.021
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