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Developing Fluorogenic Riboswitches for Imaging Metabolite Concentration Dynamics in Bacterial Cells

Genetically encoded small molecule sensors are important tools for revealing the dynamics of metabolites and other small molecules in live cells over time. We recently developed RNA-based sensors that exhibit fluorescence in proportion to a small molecule ligand. One class of these RNA-based sensors...

Ausführliche Beschreibung

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Bibliographische Detailangaben
Veröffentlicht in:Methods Enzymol
Hauptverfasser: Litke, Jacob L., You, Mingxu, Jaffrey, Samie R.
Format: Artigo
Sprache:Inglês
Veröffentlicht: 2016
Schlagworte:
Online Zugang:https://ncbi.nlm.nih.gov/pmc/articles/PMC5540731/
https://ncbi.nlm.nih.gov/pubmed/27241761
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/bs.mie.2016.03.021
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