Addressing Preferred Specimen Orientation in Single-Particle Cryo-EM through Tilting

We present a strategy for tackling preferred particle orientation in single-particle cryogenic electron microscopy (cryo-EM) by employing tilts during data collection, and we quantify the resulting directional resolution and density isotropy using a new 3D Fourier Shell Correlation (FSC) algorithm....

Disgrifiad llawn

Wedi'i Gadw mewn:
Manylion Llyfryddiaeth
Cyhoeddwyd yn:Nat Methods
Prif Awduron: Tan, Yong Zi, Baldwin, Philip R., Davis, Joseph H., Williamson, James R., Potter, Clinton S., Carragher, Bridget, Lyumkis, Dmitry
Fformat: Artigo
Iaith:Inglês
Cyhoeddwyd: 2017
Pynciau:
Article
Mynediad Ar-lein:https://ncbi.nlm.nih.gov/pmc/articles/PMC5533649/
https://ncbi.nlm.nih.gov/pubmed/28671674
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1038/nmeth.4347
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