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Addressing Preferred Specimen Orientation in Single-Particle Cryo-EM through Tilting
We present a strategy for tackling preferred particle orientation in single-particle cryogenic electron microscopy (cryo-EM) by employing tilts during data collection, and we quantify the resulting directional resolution and density isotropy using a new 3D Fourier Shell Correlation (FSC) algorithm....
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| Veröffentlicht in: | Nat Methods |
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| Hauptverfasser: | , , , , , , |
| Format: | Artigo |
| Sprache: | Inglês |
| Veröffentlicht: |
2017
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| Schlagworte: | |
| Online Zugang: | https://ncbi.nlm.nih.gov/pmc/articles/PMC5533649/ https://ncbi.nlm.nih.gov/pubmed/28671674 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1038/nmeth.4347 |
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