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High-quality frozen extracts of Xenopus laevis eggs reveal size-dependent control of metaphase spindle micromechanics

Cell-free extracts from unfertilized Xenopus laevis eggs offer the opportunity for a variety of biochemical and biophysical assays for analyzing essential cell cycle events such as metaphase spindle assembly. However, the extracts often exhibit substantial variation in quality and have low storage s...

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Pubblicato in:Mol Biol Cell
Autori principali: Takagi, Jun, Shimamoto, Yuta
Natura: Artigo
Lingua:Inglês
Pubblicazione: The American Society for Cell Biology 2017
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Accesso online:https://ncbi.nlm.nih.gov/pmc/articles/PMC5531733/
https://ncbi.nlm.nih.gov/pubmed/28592634
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1091/mbc.E17-03-0174
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