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Generation of chromosomal translocations that lead to conditional fusion protein expression using CRISPR-Cas9 and homology-directed repair

Recurrent chromosomal translocations often lead to expression of fusion proteins associated with oncogenic transformation. To study translocations and downstream events, genome editing techniques have been developed to generate chromosomal translocations through non-homologous end joining of DNA dou...

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Detalhes bibliográficos
Publicado no:Methods
Main Authors: Vanoli, Fabio, Jasin, Maria
Formato: Artigo
Idioma:Inglês
Publicado em: 2017
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC5531069/
https://ncbi.nlm.nih.gov/pubmed/28522325
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.ymeth.2017.05.006
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