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Immunoenzymatic detection of expressed gene fragments cloned in the lac Z gene of E. coli.

We describe a method which permits the detection of exon fragments. Such DNA was cloned and expressed in the promoter proximal part of the lac Z gene of Escherichia coli. The resulting antigen-beta-galactosidase chimeras are bound to their respective antibodies fixed to polyvinyl sheets. The beta-ga...

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Detalhes bibliográficos
Publicado no:EMBO J
Main Authors: Koenen, M, Rüther, U, Müller-Hill, B
Formato: Artigo
Idioma:Inglês
Publicado em: Nature Publishing Group 1982
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC553076/
https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/6329687/
https://ncbi.nlm.nih.govhttps://doi.org/10.1002/j.1460-2075.1982.tb01199.x
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