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Exon cloning: immunoenzymatic identification of exons of the chicken lysozyme gene.

A 10-kilobase DNA fragment containing exons 1 and 2 of the chicken lysozyme gene has been randomly cleaved with DNase I. After tailing and cloning into the plasmid pUK230, Lac+ colonies were selected. Colonies harboring expressed fragments of the exons could be detected by an immunoenzymatic assay u...

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Detalhes bibliográficos
Publicado no:Proc Natl Acad Sci U S A
Main Authors: Rüther, U, Koenen, M, Sippel, A E, Müller-Hill, B
Formato: Artigo
Idioma:Inglês
Publicado em: National Academy of Sciences 1982
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC347231/
https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/6184717/
https://ncbi.nlm.nih.govhttps://doi.org/10.1073/pnas.79.22.6852
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