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Probing site-specific conformational distributions in protein folding with solid-state NMR

We demonstrate an experimental approach to structural studies of unfolded and partially folded proteins in which conformational distributions are probed at a site-specific level by 2D solid-state (13)C NMR spectroscopy of glassy frozen solutions. Experiments on chemical denaturation of the 35-residu...

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Autori principali: Havlin, Robert H., Tycko, Robert
Natura: Artigo
Lingua:Inglês
Pubblicazione: National Academy of Sciences 2005
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Accesso online:https://ncbi.nlm.nih.gov/pmc/articles/PMC552907/
https://ncbi.nlm.nih.gov/pubmed/15718283
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1073/pnas.0406130102
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