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A CRISPR Resource for Individual, Combinatorial, or Multiplexed Gene Knockout

We have combined a machine-learning approach with other strategies to optimize knockout efficiency with the CRISPR/Cas9 system. In addition, we have developed a multiplexed sgRNA expression strategy that promotes the functional ablation of single genes and allows for combinatorial targeting. These s...

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Bibliografiske detaljer
Udgivet i:Mol Cell
Main Authors: Erard, Nicolas, Knott, Simon R.V., Hannon, Gregory J.
Format: Artigo
Sprog:Inglês
Udgivet: Cell Press 2017
Fag:
Online adgang:https://ncbi.nlm.nih.gov/pmc/articles/PMC5526787/
https://ncbi.nlm.nih.gov/pubmed/28732207
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.molcel.2017.06.030
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