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Nanopore long-read RNAseq reveals widespread transcriptional variation among the surface receptors of individual B cells

Understanding gene regulation and function requires a genome-wide method capable of capturing both gene expression levels and isoform diversity at the single-cell level. Short-read RNAseq is limited in its ability to resolve complex isoforms because it fails to sequence full-length cDNA copies of RN...

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Detalhes bibliográficos
Publicado no:Nat Commun
Main Authors: Byrne, Ashley, Beaudin, Anna E., Olsen, Hugh E., Jain, Miten, Cole, Charles, Palmer, Theron, DuBois, Rebecca M., Forsberg, E. Camilla, Akeson, Mark, Vollmers, Christopher
Formato: Artigo
Idioma:Inglês
Publicado em: Nature Publishing Group 2017
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC5524981/
https://ncbi.nlm.nih.gov/pubmed/28722025
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1038/ncomms16027
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