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Improving nanopore read accuracy with the R2C2 method enables the sequencing of highly multiplexed full-length single-cell cDNA

High-throughput short-read sequencing has revolutionized how transcriptomes are quantified and annotated. However, while Illumina short-read sequencers can be used to analyze entire transcriptomes down to the level of individual splicing events with great accuracy, they fall short of analyzing how t...

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Bibliografiska uppgifter
I publikationen:Proc Natl Acad Sci U S A
Huvudupphovsmän: Volden, Roger, Palmer, Theron, Byrne, Ashley, Cole, Charles, Schmitz, Robert J., Green, Richard E., Vollmers, Christopher
Materialtyp: Artigo
Språk:Inglês
Publicerad: National Academy of Sciences 2018
Ämnen:
Länkar:https://ncbi.nlm.nih.gov/pmc/articles/PMC6166824/
https://ncbi.nlm.nih.gov/pubmed/30201725
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1073/pnas.1806447115
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